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    SONG Hong-tao, JIAO Dong-liang, WANG Li-jin, MU Lin-lin, WANG Wen-juan, SONG Pei-pei, ZHAI Chang-ping, HE Zhen. Construction and analysis of recombinant plasmid pmirGLO-DISC1 3'UTR of schizophrenia susceptibility gene DISC1[J]. Journal of Bengbu Medical University, 2022, 47(1): 26-29. DOI: 10.13898/j.cnki.issn.1000-2200.2022.01.006
    Citation: SONG Hong-tao, JIAO Dong-liang, WANG Li-jin, MU Lin-lin, WANG Wen-juan, SONG Pei-pei, ZHAI Chang-ping, HE Zhen. Construction and analysis of recombinant plasmid pmirGLO-DISC1 3'UTR of schizophrenia susceptibility gene DISC1[J]. Journal of Bengbu Medical University, 2022, 47(1): 26-29. DOI: 10.13898/j.cnki.issn.1000-2200.2022.01.006
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    Construction and analysis of recombinant plasmid pmirGLO-DISC1 3'UTR of schizophrenia susceptibility gene DISC1

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      • Abstract
    • ObjectiveTo construct the recombinant plasmid of the disrupted in schizophrenia 1(DISC1) pmirGLO-DISC1 3'UTR, then analyze and identify the recombinant plasmid.
      MethodsThe binding sites of differentially expressed miRNA-181b-5p in schizophrenia and schizophrenia susceptibility gene DISC1 were analyzed by bioinformatics.The upstream and downstream 200 bp length sequence of DISC1 3'UTR (1.26 kb-WT) including the binding sites was selected and synthesized.The synthesized target gene was inserted into H343pmirGLO plasmid to construct pmirGLO-DISC1 3'UTR, which was analyzed and identified.
      ResultsThe sequencing results showed that the DISC1 3'UTR (1.26 kb-WT) gene was successfully inserted into the H343pmirGLO plasmid.The pmirGLO-DISC1 3'UTR sequence was correct.Compared with the target sequence, the query cover of pmirGLO-DISC1 3'UTR sequence was 84% and the identity was 100%.
      ConclusionsThe recombinant plasmid pmirGLO-DISC1 3'UTR is successfully constructed, which lays a foundation for the regulation of schizophrenia susceptibility gene DISC1 by miRNA-181b-5p and can be used in follow-up study.
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