Study on the relationship between homocysteine and MTHFR 677C/T gene polymorphism in patients with hypertension and evaluation on the effect of different doses of folic acid intervention

ObjectiveTo investigate the correlation between homocysteine(Hcy) level and MTHFR 677C/T gene polymorphism in patients with hypertension, and evaluate the effect of different doses of folic acid on different genotypes of hyperhomocysteinemia.

MethodsThree hundred and fifty-nine patients with hypertension were screened for Hcy screening and MTHFR 677C/T gene detection. The gene type was divided into type CC, CT and TT, and 50 patients in each gene type were randomly divided into two groups(25 cases in each group), who were treated with 0.8 mg and 5 mg folic acid for 8 weeks to evaluate the therapeutic effect.

ResultsAmong 359 hypertensive patients, 119 cases were combined with hyperhomocysteinemia, and the incidence rate was 55.43%. The incidence of hyperhomocysteinemia and plasma Hcy level in male patients were significantly higher than those in female patients(P < 0.01). The results of MTHFR 677C/T gene detection showed that among 359 patients, 161 cases were with type CC, 118 cases were with type CT, and 80 cases were with type TT. The incidence of hyperhomocysteinemia and plasma Hcy level in type TT hypertensive patients were higher than those in type CC and type CT patients(P < 0.01). There was no significant difference in plasma Hcy level between 0.8 mg group and 5 mg group of the same gene type before and after intervention(P > 0.05). The plasma Hcy level in 0.8 mg group and 5 mg group of different gene type was significantly lower than those before intervention(P < 0.01). The difference of Hcy level in different gene type in the same group after intervention was statistically significant(P < 0.01), the Hcy level of type CC in 0.8 mg group and 5 mg group was lower than that of type CT and TT(P < 0.05), and Hcy level of type CT in 0.8 mg group was lower than that of type TT(P < 0.05).

ConclusionsThe Hcy level is correlated with MTHFR 677C/T gene polymorphism.Folic acid can effectively reduce hyperhomocysteinemia. There is no significant difference between high-dose folic acid and low-dose folic acid. The effect of folic acid intervention was different with different gene types.