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    CAI Hao, GUI Zheng, YANG Zheng, LI Yan, LIU Na, HUO Harihan, BU Fan-yi, ZOU Zhen-yu, DUAN Ya-gan, LIU Tao-di. Genotype identification of Trib3 knockout rats using CRISPR/Cas9 technology[J]. Journal of Bengbu Medical University, 2022, 47(4): 437-441. DOI: 10.13898/j.cnki.issn.1000-2200.2022.04.004
    Citation: CAI Hao, GUI Zheng, YANG Zheng, LI Yan, LIU Na, HUO Harihan, BU Fan-yi, ZOU Zhen-yu, DUAN Ya-gan, LIU Tao-di. Genotype identification of Trib3 knockout rats using CRISPR/Cas9 technology[J]. Journal of Bengbu Medical University, 2022, 47(4): 437-441. DOI: 10.13898/j.cnki.issn.1000-2200.2022.04.004
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    Genotype identification of Trib3 knockout rats using CRISPR/Cas9 technology

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      • Abstract
    • ObjectiveTo construct a Trib3 knockout rat model and identify the related genotype.
      MethodsAfter targeted knockout of Trib3 gene in rat model, the deletion of exon 3 of Trib3 gene was detected, and the genotype was identified by gene sequencing and PCR methods.
      ResultsThe positive F0 generation of Trib3-/+ rats was obtained, the F1 and F2 generations were obtained by self-crossing, and the F2 generation of Trib3-/- rats was selected.
      ConclusionsTrib3 knockout rat models are successfully prepared, and Trib3-/-, Trib3-/+ and Trib3+/+ rats are successively identified and can be stably inherited, it provides a theoretical basis for subsequent experiments.
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