Expression of RIP1-RIP3-MLKL signaling pathway in peripheral blood monocytes of patients with acute coronary syndrome

ObjectiveTo investigate the expression changes of RIP1-RIP3-MLKL signaling pathway in peripheral blood mononuclear cells(PBMCs) of patients with acute coronary syndrome(ACS) and analyze the mechanism.

MethodsSixty patients with ACS were enrolled, including 31 patients with unstable angina(UAP) and 20 patients with acute myocardial infarction(AMI).The control group received 19 healthy cases.Peripheral venous blood was collected from all subjects, plasma and monocytes were extracted, and interleukin(IL)-1 and IL-18 were detected by enzyme-linked immunosorbent assay(ELISA) and turbidimetry.The level of MLKL was detected by real-time PCR.The expression of RIP1, RIP3 and MLKL in PBMCs were detected by Western blotting.

ResultsThe levels of IL-1, IL-18 and MLKL in plasma of 3 groups were significantly different(P < 0.01), RIP1 and RIP3 protein expression increased in PBMCs(P < 0.05), mRNA and protein expression of MLKL were also increased(P < 0.05).Compared with the UAP group, IL-1, IL-18 and MLKL were increased in peripheral blood of patients with AMI, and the protein expression of RIP1 and RIP3 were increased(P < 0.05).The expressions of MLKL mRNA and protein expression were also increased.

ConclusionsThere are different degrees of inflammatory activation in UAP and AMI patients.The expression of RIP1-RIP3-MLKL signaling pathway in PBMCs gradually increases with the severity of the disease, suggesting that RIP1-RIP3-MLKL signaling pathway may play an important role in ACS.