Expression of NLRP3 in secondary hyperparathyroidism and its clinical significance

ObjectiveTo investigate the expression and clinical significance of NOD-like receptor family pyrin domain containing 3(NLRP3) in secondary hyperparathyroidism(SHPT).

MethodsHE staining was used to observe the difference of parathyroid gland in normal people and SHPT patients.ELISA was used to detect the serum level of NLRP3 in normal people and SHPT patients.Immunohistochemistry of SP method was used to detect the expression of NLRP3 in normal and hyperplastic parathyroid tissue, and the relationship between NLRP3 expression and SHPT clinical-pathological characteristics was analyzed.

ResultsHyperplastic parathyroid glands in SHPT patients manifested as diffuse and adenoma-like hyperplasia, which was obviously different from that of normal, and the serum level of NLRP3 in SHPT patients was significantly higher than that of normal people(P < 0.01).The positive rate of NLRP3 in abnormal hyperplastic parathyroid tissue in SHPT patients was significantly higher than that in normal parathyroid tissue(P < 0.01).The expression of NLRP3 in SHPT patients had no correlation with gender, age, dialysis method or dialysis age(P>0.05).

ConclusionsNLRP3 may be involved in the occurrence and development of SHPT, and its high expression is expected to become an important indicator for the early diagnosis and prognostic evaluation of SHPT.