Effects of miR-367 on proliferation, migration and invasion of endometrial cancer cells by targeted regulating TET2

Objective To investigate the effect of miR-367 on the proliferation, migration and invasion of endometrial cancer cells by targeted regulating ten-eleven translocation methylcytosine dioxygenase 2(TET2).

MethodsHuman endometrial cancer HEC-1A cells were divided into 5 groups: blank group(NG), negative transfection group(mimics NC), miR-367 overexpression group(miR-367 mimics), inhibitor NC group and miR-367 inhibitor group.qRT-PCR was used to determine the expression levels of TET2 mRNA and miR-367 in HEC-1A cells; MTT assay was applied to detect cell proliferation; Transwell assay was carried out to detect cell invasion and migration ability; TargetScan database was employed to predict the target gene of miR-367, and dual luciferase reporter gene assay was used to verify the relationship; Western blotting was performed to analyze the protein expression levels of TET2, c-myc, cyclin D1, MMP-2 and MMP-9.

ResultsCompared with the NG group and mimics NC group, in the miR-367 mimics group, the expression level of TET2 mRNA in HEC-1A cells decreased(P < 0.05), the expression level of miR-367 increased(P < 0.05), the cell survival rate aftertreated for 24, 48 h and number of invaded and migrated cells increased(P < 0.05), the protein expression level of TET2 decreased(P < 0.05), the protein expression levels of MMP-2, MMP-9, c-myc and cyclin D1 increased(P < 0.05).Compared with NG group and inhibitor NC group, in the miR-367 inhibitor group, the expression level of TET2 mRNA in HEC-1A cells increased(P < 0.05), the expression level of miR-367 decreased(P < 0.05), the cell survival rate aftertreated for 24, 48 h and number of invaded and migrated cells decreased, the protein expression level of TET2 increased(P < 0.05), the protein expression level of MMP-2, MMP-9, c-myc and cyclin D1 decreased(P < 0.05).TargetScan database prediction showed that miR-367 was a potential target gene of TET2.The results of the luciferase reporter assay showed that the luciferase activity of HEC-1A cells in the miR-367 mimics+WT group was lower than that in the miR-367 NC+WT group(P < 0.05), and there was no significant difference in luciferase activity between the miR-367 NC+MUT group and miR-367 mimics+MUT group(P>0.05).

ConclusionsOverexpression of miR-367 may promote the proliferation, migration and invasion of endometrial cancer HEC-1A cells by targeted inhibiting TET2.