Study on CLEC4M in promoting the proliferation and migration of brain astroblastoma

ObjectiveTo investigate the effect of CLEC4M on brain astroblastoma proliferation and migration, as well as the mechanism behind it.

MethodsBrain astroblastoma cell lines overexpressing and interfering with CLEC4M were constructed by lentivirus transfection. Real-time quantitative PCR was used to determine the level of CLEC4M expression. The effects of CLEC4M on the proliferation and migration of brain astroblastoma were studied using the CCK-8 test and the cell scratch assay, and the effect of CLEC4M on the cell cycle of brain astroblastoma was studied using flow cytometry. Transcriptome sequencing was used to identify genes in cancer signaling pathways regulated by CLEC4M.

ResultsBrain astroblastoma cell lines overexpressing and interfering with CLEC4M were successfully constructed, and the overexpression and interference effect were significant(P < 0.01). CLEC4M increased astroblastoma proliferation and migration, and it mostly affected the G₀/G₁ and S phases of the cell cycle to regulate cell cycle progression(P < 0.05 to P < 0.01). In addition, overexpression of CLEC4M in brain astroblastoma also modified the expression levels of 5 857 genes, with 2 897 genes highly up-regulated and 2 960 genes significantly down-regulated, according to transcriptome sequencing studies. The differentially expressed genes were largely enriched in ribosomes, cell cycle, and cancer signaling pathways, according to KEGG analysis. CLEC4M interacted with CCNB1, CCND3, APAF1, STEAP3, RRM2B and FAS genes in cancer signaling pathways(P < 0.05 to P < 0.01).

ConclusionsCLEC4M can increase the proliferation and migration of brain astroblastoma.