Study on the expression of Piwil2 protein in malignant phyllodes tumor tissue and its clinical prognosis

Objective To investigate the expression levels of Piwil2 protein in breast malignant lobar tumor tissues, and analyze the relationship between Piwil2 protein expression and clinicopathological factors related to breast malignant lobar tumor and its influence on 10-year survival and prognosis of patients.

Methods The clinical data of 50 female patients with malignant lobular tumor of breast were collected, and 70 cases of normal breast tissue resected due to mastitis were selected as the control group. The expression levels of Piwil2 protein in malignant lobar tumor and normal breast tissues were detected by EnVisionTM immunohistochemistry.

Results The positive expression rate of Piwil2 protein in malignant lobar tumor tissue was 86.00%, which was significantly higher than that in normal breast tissue(0.00%)(χ²=93.82, P < 0.01). The positive expression of Piwil2 protein in malignant lobar tumor tissue was related to the tumor diameter≥10 cm, Ki-67≥25%, oncocyte atypia, CyclinD1 protein positive expression and Karyokinesis≥15/10HPF(P < 0.05 to P < 0.01), but not related to the age and postoperative recurrence(P>0.05). The ten-year survival rate of malignant lobar tumor patients with positive expression of Piwil2 protein(65.12%) was lower than that of patients with negative expression of Piwil2 protein(100.00%), and the difference of which was statistically significant(χ²=3.95, P < 0.05).

Conclusions The abnormal expression of Piwil2 protein is involved in the evolution and development of malignant breast lobar tumor, and can also be used as a reference index for predicting the survival and prognosis of patients.