ObjectiveTo investigate the gene locus of antihypertensive drug metabolizing enzymes CYP2C9*3(1075A>C), CYP2D6*10(100C>T), CYP3A5*3(6986A>G) and genotype distribution frequency of the drug target gene loci AGTR1(1166 A>C), ACE(I/D), ADRB1(1165G>C) and NPPA(2238T>C), to provide a theoretical basis for the personalized medication of five types of antihypertensive drugs including β-blocker, angiotensin receptor antagonist, angiotensin-converting enzyme inhibitor, calcium ion antagonist, and diuretics in hypertensive patients in Bengbu area of Anhui.
MethodsThe PCR melting curve method was used to detect the gene locus polymorphism of drug in 879 hypertensive patients hospitalized from January 2020 to December 2020, and the typing frequency of each gene locus was analyzed.
ResultsThe frequency of wild type, heterozygous mutant and homozygous mutant of AGTR1(1166A>C) gene locus in 879 hypertensive patients was 88.74%, 10.92% and 0.34%, respectively, which was 5.23%, 35.38% and 59.39% for ADRB1(1165G>C) gene locus, 40.73%, 43.12% and 16.15% for ACE(I/D) gene locus, 98.63 %, 1.37% and 0% for NPPA(2238T>C) gene locus, 91.81%, 7.96% and 0.23% for CYP2C9*3(1075A>C) gene locus, 25.94%, 45.73% and 28.33% for CYP2D6*10(100C>T) gene locus, and 7.17%, 39.70% and 53.13% for CYP3A5*3(6986A>G) gene locus.Among them, the distribution difference of genotype at CYP2C9*3(1075A>C) gene locus was statistically significant between male and female patients(P < 0.05), and the distribution difference of allele gene polymorphism of CYP2C9*3 and CYP2D6*10 was statistically significant between male and female patients(P < 0.05).
ConclusionsThe data on the distribution frequency of hypertensive drug-related gene polymorphisms in the primary hypertension population in Bengbu area of Anhui have been obtained, which provide a basis for the establishment of a patient gene pool, and facilitate further study on the polymorphisms of related gene locus in future to guide rational drug use in clinic.
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