Study on the regulatory effects of miR-127 on biological behavior of cutaneous quamous cell carcinoma cells

ObjectiveTo investigate the regulatory effects of miR-127 on biological behavior of skin squamous cell carcinoma cells.

MethodsThe samples of skin squamous cell carcinoma tissues and adjacent normal tissues were collected, and the expression levels of miR-127 in two groups were detected by real-time quantitative PCR.The expression level of miR-127 in squamous cell carcinoma cell line A431 was regulated to detect the changes of proliferation and invasion ability of squamous cell carcinoma cells.The targets of miR-127 were verified by bioinformatics prediction websites and molecular biological methods.

ResultsThe expression level of miR-127 in skin squamous cell carcinoma tissues was significantly higher than that in adjacent tissues(P < 0.01).Compared with HaCaT cells, the expression levels of miR-127 in A431 cells and HSC-5 cells significantly increased, and the difference of which was statistically significant(P < 0.05).After up-regulating the expression of miR-127, the proliferation and invasion ability of A431 cells were significantly enhanced(P < 0.05).The proliferation and invasion ability of A431 cells were inhibited after down-regulating the expression of miR-127(P < 0.05).The results of dual luciferase reporter gene assay showed that the miR-127 could directly bind to PTEN, and the expression level of PTEN proetin was changed after regulating miR-127(P < 0.05).

ConclusionsThe miR-127 is involved in the regulation of biological behavior of squamous cell carcinoma cells.miR-127 may be used as a potential molecular target in the treatment of skin squamous cell carcinoma.