Correlation of serum high mobility group protein B1 with renal function, inflammatory factors and oxidative stress in patients with diabetic nephropathy

ObjectiveTo observe the correlation of serum high mobility group protein B1(HMGB1) with renal function, inflammatory factors, oxidative stress of patients with diabetic nephropathy(DN).

MethodsOne hundred and seventeen patients with DN were divided into the microalbuminuria group(n=55) and the large albuminuria group(n=62) according to the 24hUPQ, and 50 patients with simple type 2 diabetes mellitus were selected as the control group during the same period.Renal function indexes serum creatinine(SCr), blood urea nitrogen(BUN), glomerlar filtration rate(GFR), cystatin C(Cys-C), inflammatory factorsnuclear factor-κB(NF-κB), tumor necrosis factor-α(TNF-α), intercellular cell adhesion molecule-1(ICAM-1), interleukin 6(IL-6) and oxidative stress indicatorsmalondialdehyde(MDA), total antioxygen capability(T-AOC), superoxide dismutase(SOD), advanced oxidation protein products(AOPP) in each group were measured, and the correlation of serum HMGB1 with renal function, inflammatory factors, oxidative stress indexes were analyzed.

ResultsThe serum HMGB1 level in the large albuminuria group and the microalbuminuria group were higher than that in the simple type 2 diabetes mellitus group(P < 0.05), while the serum HMGB1 level of patients in the large albuminuria group was higher than that in the microalbuminuria group(P < 0.05).There was a statistically significant difference in three groups between the renal indicators such as SCr, BUN, GFR, Cys-C(P < 0.01), and the SCr, BUN, Cys-C in the simple type 2 diabetes mellitus group, the microalbuminuria group and the large albuminuria group gradually increased, but the GFR gradually decrease(P < 0.01).There was a statistically significant difference in the levels of serum NF-κB, TNF-α, ICAM-1, IL-6 in three groups (P < 0.01).The levels of NF-κB, TNF-α, ICAM-1 and IL-6 in the simple type 2 diabetes mellitus group, the microalbuminuria group and the large albuminuria group gradually increased(P < 0.01).There was a statistically significant difference in three groups between the oxidative stress indicators such as MDA, T-AOC, SOD, AOPP(P < 0.01).The serum MDA and AOPP levels in simple type 2 diabetes mellitus group, microalbuminuria group and the large albuminuria group gradually increased, but the serum T-AOC and SOD levels gradually decreased(P < 0.01).Serum HMGB1 was significantly correlated with renal function, inflammatory factors and oxidative stress indicators(P < 0.05).

ConclusionsThe level of serum HMGB1 in DN patients is increased significantly with the aggravation of disease severity, which is related to the injury of renal function, inflammation and the severity of oxidative stress reaction.