miR-107 suppresses cell proliferation and invasion by targeting tumor protein D52 in prostate cancer

ObjectiveTo investigate the effects and potential mechanism of miR-107 in prostate cancer.

MethodsTransient transfection, cell cloning, cell scratching and invasion experiments were used to detect the effects of miR-107 group and control group(miR-NC) on the biological functions of prostate cancer cells.In addition, we selected tumor protein D52(TPD52) as a potential downstream target gene of miR-107 by TargetscanHuman7.2, and used dual-luciferase reporter assay for verifing the specific binding.Meanwhile, Western blotting and immunofluorescence assays were performed to detected the expression of target gene.

ResultsThe healing rate, invasion rate and proliferation rate of cell scratch in miR-107 group were lower than those in miR-NC group(P < 0.01).The expression of target gene TPD52 in miR-107 group was less than that in miR-NC group(P < 0.01).

ConclusionsmiR-107 inhibits the proliferation and invasion of prostate cancer by targeting TPD52.