ObjectiveTo investigate the killing effect of Vγ9Vδ2 T immune cells combined with cisplatin(CDDP) on nasopharyngeal carcinoma cells.
MethodsThe CCK8 method was used to observe the effect of low-concentration of CDDP and zoledronate(ZOL) combined with Vγ9Vδ2 T cells on the proliferation activity of nasopharyngeal carcinoma cells.Flow cytometry was applied to determine the secretion of tumor necrosis factor-α(TNF-α), interferon-γ(IFN-γ) and CD107a in Vγ9Vδ2 T cells, and the expression of MICA and MICB on the surface of nasopharyngeal carcinoma cells.Western blotting was employed to detect the protein expression of MICA and MICB in nasopharyngeal carcinoma cells.
ResultsThe survival rate of nasopharyngeal carcinoma CNE2Z cells decreased with the increase of CDDP concentration, the killing effect of CDDP combined with Vγ9Vδ2 T cells on CNE2Z cells was better than that of CDDP alone(P < 0.05).ZOL promoted the proliferation of Vγ9Vδ2 T cells, and the killing effect of ZOL combined with Vγ9Vδ2 T cells on CNE2Z cells increased with the increase of ZOL concentration(P < 0.05).Low-concentration of CDDP and ZOL combined with Vγ9Vδ2 T cells enhanced the killing effect on nasopharyngeal carcinoma cells, the killing effect of combined treatment was the best at the effect-target ratio of 10:1, and the difference of which was statistically significant(P < 0.05).Nasopharyngeal carcinoma cells treated with low-concentration of CDDP and ZOL could increase the secretion of immune factors CD107a, TNF-α and IFN-γ in Vγ9Vδ2 T cells(P < 0.05).Low-concentration of CDDP combined with Vγ9Vδ2 T cells significantly up-regulated the expression of MICA and MICB on the surface of nasopharyngeal carcinoma cells(P < 0.01).
ConclusionsCDDP can enhance the killing effect of Vγ9Vδ2 T cells on nasopharyngeal carcinoma cells, which may be related to the enhanced expression of MICA and MICB on the surface of nasopharyngeal carcinoma cells and the increased secretion of TNF-α and IFN-γ in Vγ9Vδ2 T cells.
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