Effects of siRNA interference with MafA on the growth of mouse islet β cells and the expression of insulin-related genes

ObjectiveTo investigate the effect of v-maf musculoaponeurotic fibrosarcoma oncogene homologue A(MafA) on the proliferation of mouse islet β cells Min6, and the mRNA and protein expression of insulin-related genes.

MethodsThe experiment was divided into MafA siRNA transfected MafA interference group A, MafA interference group B, MafA interference group C, and non-transfected control group D, control group E, control group F.MafA interference group A and control group D were treated with 5.6 mmol/L glucose, MafA interference group B and control group E were treated with 10 mmol/L glucose, and MafA interference group C and control group F were treated with 25 mmol/L glucose.The expression of mRNA and protein was detected by real-time PCR and Western blotting.The proliferation of islet cells was detected by MTT assay.The concentration of insulin was determined by ELISA.

ResultsThere were significant differences in the expression of MafA mRNA and protein between MafA interference group B and control group E, MafA interference group C and control group F(P < 0.05).The proliferation level of Min6 cells in MafA interference group C was lower than that in control group F(P < 0.05).The relative expression levels of Insulin-1 and Insulin-2 mRNA in MafA interference group C were lower than those in control group F(P < 0.05).The insulin concentration in MafA interference group C was lower than that in control group F(P < 0.05).

ConclusionsMafA gene is related to the proliferation and insulin secretion of mouse islet β cells, which may be related to the concentration of blood glucose.