Study on the mechanism of circ_0001178 targeting miR-1179 to regulate the proliferation and apoptosis of gastric cancer AGS cells

ObjectiveTo explore the mechanism of circ_0001178 targeting miR-1179 to regulate the proliferation and apoptosis of gastric cancer AGS cells.

MethodsThe expression levels of circ_0001178 and miR-1179 in 33 samples of gastric cancer tissue and adjacent tissues were detected using the real-time fluorescent quantitative PCR.The gastric cancer cell line AGS was randomly divided into the si-NC group, si-circ_0001178 group, miR-NC group, miR-1179 group, si-circ_0001178+anti-miR-NC group and si-circ_0001178+anti-miR-1179 group.The number of clone formation was detected using the clone formation experiment, and the cell viability was detected using MTT assay.The apoptosis and protein expression were detected using the flow cytometry and Western blotting, respectively.The dual luciferase reporter assay was used to detect the targeting relationship between circ_0001178 and miR-1179.

ResultsThe expression level of circ_0001178 in cancer tissue was higher than that in adjacent tissues, while the expression level of miR-1179 in cancer tissue was lower than that in adjacent tissues(P < 0.01).The inhibition of circ_0001178 expression or overexpression of miR-1179 could reduce the number of AGS cell clone formation, decrease the cell activity, increase the AGS cell apoptosis rate, and increase the expression levels of cleaved-caspase3 and cleaved-caspase9(P < 0.05).

ConclusionsInhibiting the expression of circ_0001178 may inhibit the proliferation of gastric cancer AGS cells, and induce cell apoptosis by targeting up-regulation of miR-1179.