Mechanism of lncRNA LINC00173 inhibiting the development of osteosarcoma by regulating miR-130a-5p

ObjectiveTo investigate the molecular mechanism of long-chain non-coding RNA (lncRNA) LINC00173 through regulating miR-130a-5p to mediate the development of osteosarcoma.

MethodsHuman osteosarcoma cell line MG-63 was cultured in vitro, and LINC00173 overexpression vector plasmid or empty plasmid was transfected into MG-63 cells, real-time fluorescence quantitative PCR (qPCR) was used to detect the transfection efficiency. CCK-8 and Transwell assay was used to analyze the proliferation, invasion and migration ability of MG-63 cells, dual luciferase reporter gene experiment and qPCR were used to detect the targeted regulation relationship between LINC00173 and miR-130a-5p. At the same time, LINC00173 and miR-130a-5p were overexpressed, and the proliferation, invasion and migration ability of MG-63 cells were detect.

ResultsqPCR results showed that the expression of LINC00173 in MG-63 cells in pc-LINC00173 group was significantly higher than that in Mock group (P < 0.01). After 48 and 72 hours of transfection, the cell proliferation activity of pc-LINC00173 group was lower than that of Mock group and pcDNA group (P < 0.01). Transwell results showed that the number of invading and migrating cells in pc-LINC00173 group was significantly reduced compared with Mock group (P < 0.05). There was a specific binding site between LINC00173 and miR-130a-5p. Compared with the miR-NC group, transfection of miR-130a-5p mimics could inhibit the relative luciferase activity of LINC00173-Wt cells (P < 0.01). Compared with the Mock group and the pcDNA group, the expression of miR-130a-5p in MG-63 cells in pc-LINC0173 group decreased (P < 0.01). Compared with pc-LINC00173 group and pc-LINC00173 + miR-NC group, the expression of miR-130a-5p in MG-63 cells in pc-LINC00173 + miR-130a-5p group was significantly increased, the absorbance value was significantly increased, and the number of invading and migrating cells was significantly increased (P < 0.01).

ConclusionsLINC00173 can inhibit the proliferation, invasion and migration of osteosarcoma MG-63 cells, and its mechanism may be related to the targeted inhibition of miR-130a-5p expression.