Relationship between serum MMP9 and PI3K levels and atrial fibrillation in type 2 diabetes

ObjectiveTo explore the relationship between the changes of serum matrix metalloproteinase-9(MMP9) and phosphatidylinositol-3-hydroxykinase(PI3K) levels mediated by oxidative stress and the occurrence and maintenance of atrial fibrillation (AF) in patients with type 2 diabetes mellitus(T2DM).

MethodsTwo hundred T2DM patients with AF were collected, including 62 cases of paroxysmal atrial fibrillation (PaAF), 70 cases of persistent atrial fibrillation (PeAF) and 68 cases of permanent atrial fibrillation(PerAF).Ninety patients with simple T2DM were selected as control group.The levels of MMP9, PI3K and protein expression in serum were measured by ELISA and Western blotting methods, and the correlation with the measured LAD was analyzed.MMP9, PI3K and LAD were included in Pearson correlation analysis and binary logistics regression analysis, and the factors affecting AF were obtained by binary Logistics regression analysis.

ResultsCompared with control group, the expression of MMP9 increased and the expression of PI3K decreased in PaAF, PeAF and PerAF groups.Compared with PaAF group, the expression of MMP9 in PeAF group and PerAF group increased and the expression of PI3K decreased (P < 0.01).Compared with PeAF group, the expression level of MMP9 in PerAF group increased and the expression level of PI3K decreased.Western blotting showed and there was an up-regulation trend in MMP9 and a down-regulation trend in PI3K.Pearson method was used to analyze the correlation between MMP9, PI3K and LAD.The results showed that in all experimental groups, the concentration of MMP9 was positively correlated with LAD (r=0.842), and PI3K was negatively correlated with LAD (r=0.868).Binary logistics regression analysis showed that MMP9, PI3K and LAD were risk factors for AF.

ConclusionsThe expression of MMP9 in serum of patients with T2DM complicated with AF is significantly increased, while the expression of PI3K is significantly decreased, and there is a certain correlation between MMP9 and LAD.The activation and expression of MMP9 and PI3K may be related to the occurrence of AF in patients with T2DM.