Distribution of high-risk HPV infection subtypes and its correlation with cervical lesions and cervical cancer

ObjectiveTo investigate the epidemiological characteristics of high-risk HPV infection and the correlation of high-risk human papillomavirus(HPV) and cervical precancerous lesions(CPLs) and cervical cancer(CC), for providing theoretical basis for effective using HPV vaccine and the prevention of CC in this area.

MethodsA total of 6 382 cervical exfoliated cells were collected from female patients(19-90 years old) in Huaibei Miners General Hospital.HPV genotypes were detected by PCR-reverse dot blot hybridization.The cervical smears were examined by cytopathology and biopsy guided by colposcopy.SPSS19.0 software was used to analyze the distribution of HR HPV genotypes, CPLs and CC in different age groups, and the relationship between different types of HR HPV and CPLs and CC.

ResultsThe HR HPV positive rate was 13.16%(840/6 382), containing high-risk single type(530, 8.30%), high-risk mixed type(122, 1.91%) and high-low-risk mixed type(188, 2.95%).The main genotypes of HR HPV were HPV-52, -16, and -58.The distribution of HR HPV infection, CPLs and CC were concentrated in the 41-50 age group.In addition, the distribution of LSIL and HSIL was also concentrated in the 41-50 age group, while CC was mainly distributed in the 41-60 age group.There was a statistically significant difference among the distribution of CPL and CC between HR HPV single infection and multiple infections(P < 0.05).HPV-16, -52 and -58 were the prominent types.

ConclusionsThe prevalence of HR HPV infections is mainly HPV-52, -16 and -58 in northern Anhui province.The 41-50 years old and 41-60 years old women are high-risk groups of CPLs and CC, respectively.HR HPV single subtype(especially HPV16 single subtype) infection is the major genotype that leads to CPLs and CC.