Analysis of the relationship between N-acetyltransferase 10 gene and prognosis in patients with acute myeloid leukemia

ObjectiveTo explore the relationship between N-acetyltransferase 10(NAT10) and prognosis of patients with acute myeloid leukemia(AML).

MethodsA total of 84 patients with newly treated AML(initial treatment group), 20 patients with relapsed AML(relapsed group), 20 patients with complete remission of AML(remission group) and 20 healthy people(control group) from September 2015 to January 2018 were investigated.The expression level of NAT10 mRNA in bone marrow mononuclear cells was detected by RT-PCR.The receiver operator characteristic(ROC) curve was used to analyze the value of NAT10 mRNA in the early diagnosis of AML, and the area under the curve(AUC), sensitivity and specificity were calculated.Eighty-four AML patients were divided into the high expression group and low expression group according to the expression level of NAT10 mRNA, and the survival prognosis between two groups was compared.

ResultsThe relative expression levels of NAT10 mRNA in bone marrow mononuclear cells in initial treatment group and recurrence group were higher than that in remission group and control group(P < 0.05 to P < 0.01).The AUC of NAT10 mRNA in the early diagnosis of AML was 0.827(95%CI: 0.723-0.930, P < 0.01), and the sensitivity and specificity were 83.6% and 82.6%, respectively.The results of COX multivariate analysis showed that the FLT3-ITD/TKD mutation and high expression of NAT10 mRNA were the independent influencing factors of the overall survival time and event-free survival time in AML patients(P < 0.05).The median overall survival time in the high NAT10 mRNA expression group was 4 months, which was lower than that of 10 months in low NAT10 mRNA expression group(P < 0.01).The median progression-free survival time in the group with high NAT10 mRNA expression group was 3 months, which was lower than that of 8 months in low NAT10 mRNA expression group(P < 0.01).

ConclusionsNAT10 may have some value in the early diagnosis and evaluation of AML.The high expression of NAT10 mRNA is related to the poor survival and prognosis of AML patients.