Value analysis of the serum levels of AT-Ⅲ and G6PD in the early diagnosis and prognosis of neonatal sepsis

ObjectiveTo detect the serum levels of antithrombin-Ⅲ(AT-Ⅲ) and glucose-6-phosphate dehydrogenase(G6PD) in neonatal sepsis, and analyze its value in the early diagnosis and prognosis of neonatal sepsis.

MethodsSeventy-seven children with neonatal sepsis, 50 children with bacterial infection without sepsis and 100 healthy newborns from January 2018 to January 2020 were divided into the sepsis group, infection group and control group, respectively.The serum level of AT-Ⅲ was detected using the developing substrate method, the serum level of G6PD was detected using the simple colorimetric method, the serum procalcitonin(PCT) level and white blood cell count(WBC) were determined suing the automatic biochemical analyzer, and the neonatal critical disease score(NCIS score) was recorded.The differences of the serum levels of AT-Ⅲ and G6PD among the sepsis group, infection group and control group, and between the good prognosis group and poor prognosis group were compared.The diagnostic value of serum levels AT-Ⅲ and G6PD in the neonatal sepsis and death were analyzed, and the risk factors of affecting neonatal sepsis were determined.

ResultsCompared with the control group, the serum levels of AT-Ⅲ and G6PD in the infection group and sepsis group significantly decreased, and the serum levels of AT-Ⅲ and G6PD in sepsis group were lower than those in infection group(P < 0.01).The area under the curve(AUC) of serum levels of AT-Ⅲ and G6PD in the early diagnosis of neonatal sepsis were 0.811(95%CI: 0.748-0.874) and 0.847(95%CI: 0.788-0.905), respectively.Compared with the survival group, the serum levels of PCT and WBC significantly increased, the NCIS score and serum levels of AT-Ⅲ and G6PD were significantly decreased, the differences of which were statistically significant(P < 0.05 to P < 0.01).The AUC of serum AT-Ⅲ and G6PD were 0.791(95%CI: 0.671-0.911) and 0.800(95%CI: 0.678-0.922), respectively.The results of logistic regression analysis showed that the levels of AT-Ⅲ and G6PD decreasing were the risk factors for neonatal septicemia death.

ConclusionsThe serum levels of AT-Ⅲ and G6PD increasing are closely related to the pathogenesis and poor prognosis of neonatal sepsis, and which have certain value in the early diagnosis and poor prognosis of neonatal sepsis.