ObjectiveTo investigate the relationship between the expression of p-cpi-17 (Thr38), KCa3.1 and Kr in uterine smooth muscle tissue of puerpera and postpartum hemorrhage based on RhoA/Rho signaling pathway.
MethodsA total of 80 patients of postpartum hemorrhage due to uterine inertia were selected as study group, and 80 patients without postpartum hemorrhage in the same period were selected as control group.The spontaneous contractile activity of isolated uterine smooth muscle and the contractile activity of uterine smooth muscle with Rho kinase inhibitor were measured by isometric tension test.Real-time fluorescence quantitative reverse transcription polymerase chain reaction method was used to detect the expression levels of RhoA, ROCKⅠ, and ROCKⅡmRNA in uterine smooth muscle tissues.Western blotting was used to detect the expression levels of RhoA, ROCKⅠ, ROCKⅡ, Thr38, KCa3.1, and KLF4.Pearson correlation was used to analyze the relationship between RhoA protein and uterine smooth muscle contractility and Thr38, KCa3.1, KLF4.
ResultsThe uterine smooth muscle contraction activity, contraction frequency and contraction amplitude of the study group were lower than those of the control group (P < 0.05 to P < 0.01).After Y-27632 was added, the uterine smooth muscle contraction activity was lower than before (P < 0.05).The expression levels of RhoA/Rho signaling pathway related mRNA and protein (RhoA, ROCKⅠ, ROCK Ⅱ) in the study group were lower than those in the control group (P < 0.01).The expression levels of Thr38, KCa3.1, and KLF4 in the study group were lower than those in the control group (P < 0.01).Pearson correlation analysis showed that RhoA/Rho signaling pathway related proteins and contractile activity were positively correlated with Thr38, KCa3.1, and KLF4 expression levels (P < 0.05).
ConclusionsThe RhoA/Rho signaling pathway and expression of Thr38, KCa3.1, and KLF4 decrease in uterine smooth muscle tissue of parturients with postpartum hemorrhage due to uterine asthenia, and are positively correlated with the decrease of contractility, and jointly participate in the occurrence of postpartum hemorrhage.