Expression of AP4 in human glioma tissue and its effect on the proliferation, migration and invasion of glioma cells

ObjectiveTo investigate the expression of activating enhancer binding protein 4(AP4) in human glioma tissue and its effect on the proliferation, migration and invasion of glioma cells.

MethodsTumor and adjacent tissues from 29 patients with gliomas were collected.The expression of AP4 in glioma tissues and adjacent tissues were measured by immunohistochemistry, RT-qPCR and Western blotting.Further, the AP4 knockout U251 and U87 cell lines were constructed using small interfering RNA(siRNA).The effect of AP4 knockdown on the proliferation, migration and invasion of glioma cells were detected by CCK-8, wound healing and Transwell test.

ResultsThe positive expression rate of AP4 protein in glioma was 72.41%(21/29), which was significantly higher than that in adjacent tissues 31.03%(9/29)(P < 0.01).The expression levels of AP4 mRNA and protein in grade Ⅰ+Ⅱ and Ⅲ+Ⅳ gliomas were significantly higher than those in adjacent tissues(P < 0.01), and the expression levels in grade Ⅲ+Ⅳ gliomas were higher than those in grade Ⅰ+Ⅱ(P < 0.05).After AP4 knockout, the proliferation, migration and invasion of U251 and U87 cells decreased significantly(P < 0.01).

ConclusionsThe expression level of AP4 is aberrant upregulated in glioma tissues and cells, and is closely related to the proliferation, migration and invasion of glioma cells.It can be a potential reference index for the poor prognosis.