Effect of shRNA SIRT3 gene on invasive ability of breast cancer cell MCF-7 and its mechanism

ObjectiveTo explore the effect of shRNA SIRT3 gene on invasive ability of breast cancer cell MCF-7 and its mechanism.

MethodsHuman breast cancer cell line MCF-7 cells were used as experimental subjects.According to the treatment method, breast cancer MCF-7 cells were divided into three groups: blank control group(breast cancer MCF-7 cells without lentivirus transfection), negative control group(breast cancer MCF-7 cells transfected with disordered sequence lentivirus vector) and shRNA interference group(breast cancer MCF-7 cells transfected with shRNA SIRT3 sequence lentivirus vector).The expression levels of SIRT3 and apoptosis-related factors caspase-3 and caspase-9 were detected by RT-PCR and Western blotting, and the invasive ability of cells was detected by Transwell kit.

ResultsThe relative expression of SIRT3 mRNA in the shRNA interference group was lower than that in the negative control group and the blank control group, and the relative expression of caspase-3 mRNA and caspase-9 mRNA was higher than those in the negative control group and the blank control group(P < 0.01).The protein expression levels of SIRT3, caspase-3 and caspase-9 detected by Western blotting showed the same trend.The results of the Transwell experiment showed that the number of migrated cells in the shRNA interference group was less than that in the negative control group and the blank control group(P < 0.01).

ConclusionsThe lentivirus interference vector shRNA-SIRT3 can promote the apoptosis of human breast cancer cells and inhibit the invasion of human breast cancer cells.