LncRNA CRNDE regulates oxygen and glucose deprivation-induced rat cortical neuron injury by targeting miR-212-5p

ObjectiveTo investigate the effect and mechanism of lncRNA CRNDE on oxygen and glucose deprivation (OGD)-induced rat cortical neurons injury.

MethodsRat cortical neurons were cultured and divided into Con group, OGD group, OGD+si-NC group, OGD+si-CRNDE group, OGD+miR-NC group, OGD+miR-212-5p group, OGD+si-CRNDE+anti-miR-NC group and OGD+si-CRNDE+anti-miR-212-5p group. The expression levels of CRNDE and miR-212-5p in the cells were detected by RT-qPCR, the cell survival rate was detected by CCK-8 method, lactic dehydrogenase(LDH) leakage rate in the cell culture supernatant was detected by the related kit, the apoptosis rate was detected by flow cytometry, and the expression of Bcl-2 and Bax proteins was detected by Western blotting. The dual luciferase reporter gene experiment was used to verify the regulatory relationship between CRNDE and miR-212-5p.

ResultsCompared with the Con group, the CRNDE level, LDH leakage rate, apoptosis rate, and Bax protein level in the OGD group were increased (P < 0.01), but miR-212-5p level, cell survival rate, and Bcl-2 protein level were decreased (P < 0.01). Compared with the OGD+si-NC group, the cell survival rate and Bcl-2 protein level in the OGD+si-CRNDE group were increased (P < 0.01), while LDH leakage rate, apoptosis rate and Bax protein level were decreased (P < 0.01). Compared with the OGD+miR-NC group, the cell survival rate and Bcl-2 protein level in the OGD+miR-212-5p group were increased (P < 0.01), while LDH leakage rate, apoptosis rate and Bax protein level were decreased (P < 0.01). Compared with the OGD+si-CRNDE+anti-miR-NC group, the cell survival rate and Bcl-2 protein level in the OGD+si-CRNDE+anti-miR-212-5p group were decreased (P < 0.01), meanwhile, LDH leakage rate, apoptosis rate and Bax protein level were increased (P < 0.01). CRNDE negatively regulated the expression of miR-212-5p.

ConclusionsInterfering CRNDE expression can promote the proliferation of OGD-induced rat cortical neurons and inhibit apoptosis and LDH leakage rate by up-regulating miR-212-5p, which reduces neuron injury.