ObjectiveTo investigate the expression of non-SMC condensin Ⅰ complex subunit H (NCAPH) in breast cancer and its impact on the migration and invasion of breast cancer cells.
MethodsThe TCGA database was used to obtain the original data, which was then analyzed to compare the expression of NCAPH in breast cancer tissue and normal breast tissue.The study also utilized the GEO database for verification purposes.The study involved an analysis of NCAPH protein expression in the HPA database.The role of NCAPH in the prognosis of breast cancer patients was analyzed using the Kaplan-Meier database.Interference fragments of the NACPH gene were screened and the effects of knocking down the NCAPH gene on the migration and invasion ability of breast cancer cells were evaluated using scratch and Transwell assays.
ResultsBoth TCGA and GEO database indicated that NCAPH was highly expressed in breast cancer tissues (P < 0.01).In contrast, the HPA database revealed that NCAPH protein levels were low in normal breast tissues, but significantly elevated in breast cancer tissues.Additionally, Kaplan-Meier database demonstrated that high expression of NCAPH was associated with a reduce survival rate in breast cancer patients(P < 0.01).There were significant differences in NCAPH mRNA expression levels between breast cancer cell lines SKBR-3, MDA-MB-231, and MCF-7 and human normal breast epithelial cell line MCF-10A (P < 0.05).The results of both scratch and Transwell assays demonstrated that interference with NCAPH significantly inhibited the migration and invasion ability of breast cancer cells (P < 0.01).
ConclusionsNCAPH is highly expressed in breast cancer tissues, and correlated with the prognosis of breast cancer patients.Knocking down NCAPH can significantly inhibit the migration and invasion of breast cancer.
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