ObjectiveTo investigate the effects of miR-141 targeting zinc finger E-box binding homeobox 2 (ZEB2) on the proliferation, migration, and invasion of glioma cells.
MethodsHuman glioma cell line U87 was cultured in vitro, transfected, and then divided into blank control (NG) group, negative transfection (mimics-NC) group, and overexpression miR-141 (miR-141-mimics) group, co-transfection (miR-141-mimics+pc-ZEB2) group.Real-time fluorescence quantitative PCR was used to detect the levels of miR-141 and ZEB2 mRNA; the proliferation, migration and invasion of U87 cells in each group were detected; Western blotting was used to detect the expression of proliferation cell nuclear antigen (PCNA), invasion and migration-related proteins (E-cadherin, N-Cadherin, Vimentin); the targeting relationship between miR-141 and ZEB2 was predicted by TargetScan database and verified by dual luciferase reporter gene experiment.
ResultsU87 cells were successfully transfected with mimics-NC, miR-141-mimics, and pc-ZEB2;compared with the NG group and the mimics-NC group, the U87 cell proliferation inhibition rate and E-Cadherin protein expression in the miR-141-mimics group were significantly increased (P < 0.05), and the clone formation rate, scratch healing rate, number of invasive cells, the expression of ZEB2 mRNA and protein, and the expression of PCNA, N-Cadherin, and Vimentin protein were reduced (P < 0.05);compared with the miR-141-mimics group, the U87 cell proliferation inhibition rate and E-Cadherin protein expression in the miR-141-mimics+pc-ZEB2 group were significantly reduced (P < 0.05), and the clone formation rate, scratch healing rate, number of invasive cells, the expression of ZEB2 mRNA and protein, and the expression of PCNA, N-Cadherin, and Vimentin protein were increased (P < 0.05).
ConclusionsOverexpression of miR-141 may target and down-regulate ZEB2 to inhibit the proliferation, invasion, and migration of glioma U87 cells.
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