ObjectiveTo observe the expression of long non-coding RNA small nucleolar RNA host gene 5 (lncRNA SNHG5) and miR-132-3p in placental tissue of preeclampsia (PE) patients, and to analyze the correlation between them, and their diagnostic value for PE.
MethodsA total of 112 patients with PE were selected as the study subjects, and divided into severe group (n=58) and mild group (n=54) according to the severity of PE.Sixty healthy pregnant women in the same period were selected as the control group.Real-time fluorescence quantitative PCR was used to detect the expression of lncRNA SNHG5and miR-132-3p in placental tissue.Pearson correlation analysis was applied to observe the correlation between lncRNA SNHG5 and miR-132-3p in placental tissue of PE patients, and the correlation between lncRNA SNHG5, miR-132-3p and clinical indicators.ROC curve was employed to analyze the diagnostic value of lncRNA SNHG5 and miR-132-3p levels for PE.
ResultsThe level of 24 h urinary protein, systolic blood pressure and diastolic blood pressure of PE patients in the mild group and severe group was higher than that in the control group (P < 0.05), and the level of systolic blood pressure and diastolic blood pressure in the severe group was higher than that in the mild group (P < 0.05).The level of lncRNA SNHG5 in the mild group and severe group was lower than that in the control group (P < 0.05), and the level of miR-132-3p was higher than that in the control group (P < 0.05);the level of lncRNA SNHG5 in the severe group was lower than that in the mild group (P < 0.05).The expression of lncRNA SNHG5 and miR-132-3p in placental tissue was correlated with 24 h urine protein, systolic blood pressure and diastolic blood pressure of PE patients (P < 0.05 to P < 0.01), but was not correlated with age, gestational week and body mass index (P>0.05).There was a negative correlation between lncRNA SNHG5 and miR-132-3p expression in placental tissue of PE patients (r=-0.509, P < 0.05).The results of ROC curve analysis showed that the area under the curve of lncRNA SNHG5 and miR-132-3p in placental tissue in the diagnosis of PE was 0.865 and 0.883, the cutoff value was 0.93 and 1.44, the sensitivity was 87.9% and 79.3%, and the specificity was 71.7% and 83.3%, respectively; the area under the curve of the combination of lncRNA SNHG5 and miR-132-3p in the diagnosis of PE was 0.921, the sensitivity and specificity were 89.7% and 81.7%, respectively.
ConclusionsLncRNA SNHG5 is lowly expressed and miR-132-3p is highly expressed in the placenta tissue of PE patients, and they are negatively correlated, which provides new ideas for the diagnosis and targeted therapy of PE.
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