ObjectiveTo investigate whether interleukin-34 (IL-34) can affect the proliferation and migration of stem cells from apical papilla (SCAPs) by the regulation of nuclear factor-κB (NF-кB).
MethodsThe enzymatic digestion method was used to culture SCAPs from the apical papillae of mice with medium incisors.The expression of CD44, CD45, CD90 and CSF-1R on the surface of SCAPs was identified by flow cytometry.SCAPs were divided into 4 groups including blank control group, IL-34 group (100 ng/mL IL-34), CSF-1R group (100 ng/mL IL-34+25 ng/mL anti-CSF-1R) and NF-κB group (100 ng/mL IL-34 + 1 μmol/L BMS-345541).Western blotting was used to analyze the relative expression of p-IкBα, IкBα, p-P65 and P65 protein in the cytoplasm and nucleus of SCAPs at 30, 60 and 120 minutes in each group.Transwell assay was performed to analyze the migration ability of SCAPs in each group.IL-34 concentration was changed to 50 ng/mL in each group, and CCK-8 was used to analyze the change of proliferation of SCAPs in each group.
ResultsFlow cytometry identified that SCAPs expressed CD44, CD90 positive and CD45 negative, and CSF-1R expressed on the surface of SCAPs.Compared with the control group, the relative expression of p-IкBα, p-P65 in the cytoplasm and p-P65 in the nucleus of SCAPs treated with IL-34 was significantly increased and the proliferation and migration ability of SCAPs were enhanced.Compared with the IL-34 group, the proliferation and migration of SCAPs were decreased in the CSF-1R and NF-кB group, and the expression of p-IкBα, p-P65 in the cytoplasm and p-P65 protein in the nucleus of SCAPs was significantly reduced.
ConclusionsIL-34 can regulate NF-κB signaling pathway in SCAPs and regulate the proliferation and migration of IL-34 through NF-κB signaling pathway.
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