Protective effect of abdominal paracentesis drainage against liver injury in rats with severe acute pancreatitis

ObjectiveTo investigate the effect of abdominal paracentesis drainage (APD) against liver injury in rats with severe acute pancreatitis (SAP).

MethodsThirty SD rats were randomly divided into SAP untreated group (SAP group), SAP APD group (APD group) and sham operation group (SHAM group), with 10 rats in each group.Sodium taurocholate was used to induce SAP model, ELISA method was used to detect the expression of tumor necrosis factor- α (TNF-α), interleukin (IL)-6 and IL-10 in rat peripheral blood; Western blotting was used to detect protein expression levels of TLR4, p-IκB, IRAK-4, TRAF6 and NF-κB p65 in liver tissue; morphology of liver tissue under microscope was observed.

ResultsThe liver histopathological results showed that the degree of liver tissue damage in the APD group was lighter than that of the SAP group and heavier than that of the SHAM group.In peripheral blood, compared with SHAM group, the expression levels of TNF-α, IL-6 and IL-10 in APD group were significantly increased (P < 0.01);compared with SAP group, the expression levels of TNF-α and TL-6 in APD group decreased (P < 0.01), and the expression level of IL-10 increased significantly (P < 0.01).In liver tissue, compared with SHAM group, the expression levels of TLR4, p-IκB, IRAK-4, TRAF6 and NF-κB p65 protein in APD group increased significantly (P < 0.01);compared with SAP group, the expressions level of TLR4, p-IκB, IRAK-4, TRAF6 and NF-κB p65 protein in APD group decreased significantly (P < 0.01).

ConclusionsEarly application of APD in rats with SAP combined with liver injury can reduce liver cell damage, and APD inhibits liver damage in SAP rats may be achieved by inhibiting the expression of TLR4/NF-κB signaling pathway.