Effect of electrospun nanofibers on wound healing in diabetic mice

ObjectiveTo investigate the effect of electrospun nanofibers on wound healing in diabetic mice models.

MethodsThe wound model in diabetic mice was established.The observation group was treated with electrospun nanofibers, the control group was treated with medicated gauze, and the normal group did not receive any treatment.Healing status was observed and wound healing rate was calculated.The mRNA levels of interleukin (IL)-1β, IL-4, IL-6, and tumor necrosis factor-α were quantified by qRT-PCR.The protein levels of epidermal growth factors (EGF), epidermal growth factor receptors (EGFR), hypoxic induction factors (HIF) -1α, and proliferating cell nuclear antigens in the wound were quantified with Western blotting.The effects of electrospun nanofibers on the proliferation and migration of human umbilical vein endothelial cells (HUVEC) were evaluated with the CCK-8 and scratch test, respectively.

ResultsCompared with the control group and the normal group, electrospun nanofibers significantly improved wound healing rate, inhibited inflammatory cytokines and increased EGF and EGFR levels in the mice model (P < 0.05 to P < 0.01).After the treatment of electrospun nanofibers, the migration and proliferative capacity of HUVEC were enhanced (P < 0.05 and P < 0.01), and the apoptosis rate was decreased (P < 0.05).

ConclusionsElectrospun nanofibers can significantly improve the healing of diabetic wound, which has potential clinical application value.