Expression of LINC01605 in oral squamous cell carcinoma and its influences on cell invasion and migration

ObjectiveTo investigate the expression of LINC01605 in oral squamous cell carcinoma and its influences on cell invasion and migration.

MethodsForty oral squamous cell carcinoma tissues and corresponding paracancerous tissue specimens from patients with oral squamous cell carcinoma were collected, and the relationship between the expression of LINC01605 in oral squamous cell carcinoma tissues and clinicopathological characteristics was analyzed.Oral squamous cell carcinoma CAL-27 cells in logarithmic phase was randomly separated into: CK group (normal culture), si-NC group (transfected with si-NC), si-LINC01605 group (transfected with si-LINC01605), pcDNA group (transfected with pcDNA), and pcDNA-LINC01605 group (transfected with pcDNA-LINC01605).QRT-PCR was applied to detect LINC01605 expression in tissues and cells; CCK-8 assay was applied to detect cell proliferation; plate cloning assay was used to detect the ability of cells to form clones; Transwell assay was applied to detect cell invasion; scratch assay was applied to detect cell migration; Western blotting was applied to detect the expression of proliferating cell nuclear antigen (PCNA), matrix metalloproteinase (MMP)-2, MMP-9, neural cadherin (N-cadherin), and epithelial cadherin (E-cadherin) in cells; and in vivo xenograft assay was used to detect tumor growth.

ResultsThe expression of LINC01605 in oral squamous cell carcinoma tissue was greatly higher than that in adjacent tissue (P < 0.01).The expression of LINC01605 was related to TNM stage, degree of differentiation and lymph node metastasis (P < 0.05 to P < 0.01).Compared with CK group and si-NC group, the obsorbance value, clone formation rate, number of invasive cells, scratch healing rate, PCNA, MMP-2, MMP-9, N-cadherin protein expression, and the mass of transplanted tumor in CAL-27 cells in the si-LINC01605 group decreased (P < 0.01), while the expression of E-cadherin protein increased (P < 0.01).Compared with CK group and pcDNA group, the change trend of corresponding indexes in CAL-27 cells in pcDNA-LINC01605 group was opposite to the above (P < 0.01).

ConclusionsLINC01605 is highly expressed in oral squamous cell carcinoma tissues, and silencing LINC01605 can inhibit the invasion and migration of CAL-27 cells.