miR-223-3p promotes the up-regulation of LncRNA ADAMTS9-AS2 expression by targeting TGFBR3 to inhibit the proliferation and migration of lung cancer cells

ObjectiveTo investigate the effect of miR-223-3p on the up-regulation of long non-coding RNA(LncRNA) ADAMTS9-AS2 by targeting transforming growth factor-β type Ⅲ receptor(TGFBR3) to inhibit the proliferation and migration of lung cancer cells.

MethodsThe mRNA expression levels of miR-223-3p and TGFBR3 in lung cancer H1299 cells were detected by RT-PCR, the protein expression level of TGFBR3 was detected by Western blotting, the expression level of LncRNA ADAMTS9-AS2 was detected by RT-qPCR, the cell proliferation ability was detected by CCK-8, and the cell migration ability was detected by Transwell migration assay and scratch assay.MiR-223-3p mimic(overexpression group), inhibitor(inhibition group) and control plasmid(control group) were transfected into H1299 cells by liposome.The expression levels of miR-223-3p, TGFBR3, LncRNA ADAMTS9-AS2, cell proliferation and cell migration were compared between before and after transfection.

ResultsCompared with before transfection, the expression levels of miR-223-3p mRNA and LncRNA ADAMTS9-AS2 in H1299 cells after transfection in the overexpression group increased(P < 0.05), the expression levels of TGFBR3 protein and mRNA decreased(P < 0.01 and P < 0.05), and the cell proliferation and migration ability decreased(P < 0.05);the expression levels of miR-223-3p mRNA and LncRNA ADAMTS9-AS2 in the inhibition group decreased(P < 0.05), the expression levels of TGFBR3 protein and mRNA increased(P < 0.01 and P < 0.05), and the cell proliferation and migration ability increased(P < 0.05).After 72 h of transfection, the expression level of TGFBR3 protein and mRNA, and the cell proliferation and migration ability were as follows: inhibition group>observation group>overexpression group(P < 0.01).The expression levels of miR-233-3p and LncRNA ADAMTS9-AS2 mRNA gradually decreased, Which were as follows: inhibition group<control group<overexpression group(P < 0.01).

ConclusionsMiR-223-3p inhibits the proliferation and migration of lung cancer H1299 cells, the mechanism of which may involve the targeted down-regulation of TGFBR3 and up-regulation of LncRNA ADAMTS9-AS2 expression.