Expression and localization of SIRT2 in the 1-cell stage of mouse zygote

ObjectiveTo investigate the expression and subcellular localization of sirtuins 2(SIRT2) in the mouse 1-cell stage zygote.

MethodsThe mRNA and protein expression profiles of SIRT2 in the development of mouse 1-cell stage zygote were detected by qRT-PCR and Western blotting.The co-localization of SIRT2 and α-tubulin was observed by cell immunofluorescence method.

ResultsIn the mouse 1-cell stage zygote, the expressions of SIRT2 at mRNA level had consistency with protein level.The expression level was increased during the transition from G₁ phase to G₂ phase, and decreased during the transition from G₂ phase to M phase.During the transition from G₂ phase to M phase, the localization of SIRT2 was gradually shifted from the cell cortex to the nucleus and localized in the spindle in the mid-M phase, when in the late M phase, it relocated to the cell cortex.

ConclusionsThe expression of SIRT2 protein in mouse 1-cell stage zygote is cell cycle phase-dependent.The expression of SIRT2 protein increases in the cytoplasm of mouse 1-cell stage zygote mitotic interphase (G₁, S, G₂), and the accumulated SIRT2 regulates the dynamics of spindle microtubules under some certain condition and plays a role in the G₂/M transition.