ObjectiveTo analyze the correlation of serum interleukin 33 (IL-33), tumor necrosis factor α (TNF-α) levels and nuclear factor κB (NF-κB) protein expression in peripheral blood lymphocytes with the severity of coronary heart disease (CHD), and explore its role and value in the development of CHD.
MethodsA total of 167 patients (CHD group) admitted to Department of Cardiology with coronary angiography and confirmed as CHD were selected, and during the same period, 27 cases with normal coronary angiography were confirmed as control group(CON group).According to Gensini score, 167 CHD patients were divided into mild lesion group(< 30 points, LS group, 84 cases), moderate lesion group(≥30 points to < 60 points, MS group, 54 cases), and severe lesion group(≥60 points, HS group, 29 cases).Serum IL-33 and TNF-α levels were detected by ELISA, the differences of IL-33 and TNF-α levels in different degrees of coronary artery disease were analyzed, and the correlation between IL-33, TNF-α levels and CHD was analyzed.Meanwhile, the peripheral blood lymphocytes in CHD group and CON group were collected for protein extraction, and NF-κB protein expression was detected by Western blotting.
ResultsSerum total cholesterol(TC) level in HS group was increased compared with that in CON group, LS group and MS group(P < 0.05), serum IL-33 and TNF-α levels in HS group were increased compared with those in MS group, LS group and CON group, and these parameters were gradually increased with the aggravation of coronary artery disease(P < 0.01).There was a positive correlation between serum IL-33, TNF-α levels and coronary artery disease(r=0.663, P < 0.01).The NF-κB protein expression in CHD group was significantly higher than that in CON group(P < 0.01).
ConclusionsWith the increasing degree of coronary artery disease, serum IL-3 and TNF-α levels were gradually increased, and there was a certain degree of linear correlation.NF-κB protein expression in CHD group was significantly increased compared with that in CON group.
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