Study on the correlation between blood concentration of risperidone and thyroid function indexes

ObjectiveTo establish a method for the determination of blood concentration of risperidone (RIS), and to explore the correlation between blood concentration of RIS and thyroid function indexes in patients with schizophrenia.

MethodsThe plasma samples were precipitated with acetonitrile, and then determined by ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) with clomipramine as the internal standard.The chromatographic column was BEH C₁₈, and the mobile phase was acetonitrile-water (containing 5 mmol/L ammonium acetate solution and 0.01% ammonia) with gradient elution.The flow rate was 0.5 mL/min, the column temperature was 40 ℃, and the injection volume was 2 μL.The methodology of UPLC-MS/MS was investigated by multiple reaction monitoring scanning, and it was used to determine the plasma RIS concentration in 116 patients with schizophrenia.The hospitalization information of related cases was collected, and the correlation between RIS blood concentration and thyroid function indexes was explored.

ResultsRIS and 9-OHRIS had a good linear relationship in the concentration range of 0.4-120.0 ng/mL.The intra-day and inter-day accuracy, extraction recovery, matrix effect and stability met the requirements.The mean steady-state plasma concentration was (49.76±18.07)ng/mL.There was no significant difference in the steady-state plasma concentration of patients at each time point of treatment (P>0.05).Except for TSH (P>0.05), the other four indicators showed a fluctuating upward trend over time after treatment (P < 0.05), but the RIS plasma concentration of female patients was positively correlated with T4, FT3 and FT4 (r=0.480, 0.524, 0.454, P < 0.05).

ConclusionsThe method is accurate, simple, rapid, sensitive and reproducible, and can be used to determine the plasma concentration of RIS in patients with schizophrenia.Higher blood drug concentration in female patients may affect the thyroid function index, and then affect the thyroid function of patients, which needs clinical attention.