Imaging study on the ⁹⁹Tc^m-DTPA-CGRRAGGSC scintigraphy in the nude mice model with non-small cell lung cancer

Objective To explore the imaging of scintigraphy of CGRRAGGSC labeled with ⁹⁹Tc^m in nude mice with non-small cell lung cancer.

Methods The DTPA-CGRRAGGSC was labeled with ⁹⁹Tc^m, and the labeling rate and stability were measured.The mice model with non-small cell lung cancer were established.The markers were injected into the tail vein of the tumor-bearing mice, the SPECT static imaging was performed after 2 hours, and the ratio of target to non-target(T/NT) was calculated.

Results The ⁹⁹Tc^m-DTPA-CGRRAGGSC was successfully radiolabeled, and the radiochemical purity of which was above 90% when it was placed in PBS and human serum at 37 ℃ for 6 h.The T/NT ratio was as high as 3.5 after 2 hours of injection of the marker through the tail vein of tumor-bearing mice.

Conclusions The ⁹⁹Tc^m-labeled DTPA-CGRRAGGSC is easy to be prepared, and it has high targeting to nude mice with non-small cell cancer.⁹⁹Tc^m-DTPA-CGRRAGGSC may play an important role in molecular imaging of non-small cell lung cancer with high expression of IL-11Rα.