Citation: | JIANG Lei, FANG Xinggang, LAN Peimin, CHEN Hanyu. Correlation analysis of serum HE4 and PS-PLA1 expression with disease activity and disease outcome in patients with rheumatoid arthritis[J]. Journal of Bengbu Medical University, 2024, 49(11): 1463-1466, 1471. DOI: 10.13898/j.cnki.issn.1000-2200.2024.11.012 |
To investigate the correlation of serum human epididymal protein 4 (HE4) and phosphatidylserine-specific phospholipase A1 (PS-PLA1) expression levels with disease activity and disease outcome in patients with rheumatoid arthritis (RA).
A total of 112 active RA patients were selected as the RA group, and divided into low activity group, moderate activity group and high activity group according to the disease activity score 28-joints (DAS28).In addition, 110 healthy examinees were taken as the control group.Chemiluminescence was used to detect the level of serum HE4, ELISA was applied to detect the level of serum PS-PLA1, the general information and serum index levels of the patients were collected, Pearson method was used to analyze the correlation between serum HE4, PS-PLA1 and clinical indicators in RA patients, Spearman method was used to analyze the correlation between serum HE4, PS-PLA1 and outcome, and ROC curve was used to analyze the diagnostic value of HE4 and PS-PLA1 in RA.
Compared with the control group, the erythrocyte sedimentation rate, serum C-reactive protein, rheumatoid factor, anti-cyclic citrullinated peptide antibody, HE4 and PS-PLA1 of patients in the RA group were obviously increased (P < 0.01).Compared with the low activity group, the levels of serum HE4 and PS-PLA1 in the moderate activity group and high activity group were increased (P < 0.05);compared with the moderate activity group, the levels of serum HE4 and PS-PLA1 in the high activity group were increased (P < 0.05).The area under the curve (AUC) of serum HE4 for diagnosing RA was 0.851 (95%CI: 0.797-0.895), with a sensitivity of 76.79% and a specificity of 82.73%;the AUC of PS-PLA1 in diagnosing RA was 0.818 (95%CI: 0.761-0.866), with a sensitivity of 77.68% and a specificity of 72.73%;the AUC of HE4 combined with PS-PLA1 diagnosis for RA was 0.903 (95%CI: 0.856-0.939), with a sensitivity of 86.61% and a specificity of 83.64%.Compared with before treatment, the levels of serum HE4 and PS-PLA1 were obviously decreased, the morning stiffness time and joint tenderness were obviously decreased, the VAS score and DAS28 score were obviously decreased after treatment (P < 0.01).Correlation analysis showed that the levels of serum HE4 and PS-PLA1 in RA patients were significantly positively correlated with erythrocyte sedimentation rate (ESR), serum C-reactive protein (CRP), rheumatoid factor (RF), anti-cyclic citrullinated peptide (CCP) antibody, DAS28 score and outcome (P < 0.01).
Serum HE4 and PS-PLA1 levels in RA patients are elevated, which are related to the degree of RA disease activity and outcome, and have certain reference values for RA diagnosis, disease evaluation, and outcome evaluation.
[1] |
CUSH JJ. Rheumatoid arthritis: early diagnosis and treatment[J]. Med Clin North Am, 2021, 105(2): 355. doi: 10.1016/j.mcna.2020.10.006
|
[2] |
杜凌燕, 张明娇, 刘鹏飞, 等. 类风湿关节炎患者血清趋化因子配体20与疾病严重程度的相关性研究[J]. 中华预防医学杂志, 2021, 55(2): 226.
|
[3] |
GONG Z, HAN S, ZHANG C, et al. Value of serum miR-21, HE4 and CA125 in surveillance for postoperative recurrent or metastatic ovarian cancer[J]. Pak J Med Sci, 2022, 38(4): 939.
|
[4] |
洪宏海, 王征, 林丽英. 人附睾蛋白4在类风湿关节炎滑膜细胞中的作用及机制[J]. 检验医学与临床, 2018, 15(14): 2039.
|
[5] |
张绍君, 李俊巧, 张永刚, 等. 系统性红斑狼疮患者血清PS-PLA1、CCL19水平与疾病活动度及免疫功能的相关性分析[J]. 疑难病杂志, 2020, 19(11): 1143.
|
[6] |
中华医学会风湿病学分会. 2018中国类风湿关节炎诊疗指南[J]. 中华内科杂志, 2018, 57(4): 242.
|
[7] |
SALAFFI F, DI CARLO M, CAROTTI M, et al. The subjective components of the Disease Activity Score 28-joints (DAS28) in rheumatoid arthritis patients and coexisting fibromyalgia[J]. Rheumatol Int, 2018, 38(10): 1911. doi: 10.1007/s00296-018-4096-z
|
[8] |
石秀芳, 吕礼应, 张玉慧, 等. 类风湿关节炎患者血清25-羟维生素D和白蛋白/纤维蛋白原比值的临床意义[J]. 中华检验医学杂志, 2022, 45(2): 186.
|
[9] |
LIN YJ, ANZAGHE M, SCHVLKE S. Update on the pathomechanism, diagnosis, and treatment options for rheumatoid arthritis[J]. Cells, 2020, 9(4): 880. doi: 10.3390/cells9040880
|
[10] |
BEHROUZI R, BARR CE, CROSBIE EJ. HE4 as a biomarker for endometrial cancer[J]. Cancers (Basel), 2021, 13(19): 4764. doi: 10.3390/cancers13194764
|
[11] |
ZHAN Y, CHEN J, WU J, et al. Human epididymis protein 4 aggravates airway inflammation and remodeling in chronic obstructive pulmonary disease[J]. Respir Res, 2022, 23(1): 120. doi: 10.1186/s12931-022-02040-7
|
[12] |
CHEN J, SUN F, BAO H, et al. Elevated serum human epididymis protein 4 is associated with disease activity and systemic involvements in primary Sjögren's syndrome[J]. Front Immunol, 2021, 12(1): 1.
|
[13] |
LIN T, XU S, WANG Y, et al. Human epididymis protein 4 as a new diagnostic biomarker for rheumatoid arthritis-associated interstitial lung disease[J]. Clin Exp Rheumatol, 2022, 40(11): 2167.
|
[14] |
JAMES NE, EMERSON JB, BORGSTADT AD, et al. The biomarker HE4(WFDC2) promotes a pro-angiogenic and immunosuppressive tumor microenvironment via regulation of STAT3 target genes[J]. Sci Rep, 2020, 10(1): 8558. doi: 10.1038/s41598-020-65353-x
|
[15] |
GAO X, CHEN D, HU X, et al. PLA1A participates in the antiviral innate immune response by facilitating the recruitment of TANK-binding kinase 1 to mitochondria[J]. J Innate Immun, 2018, 10(4): 315. doi: 10.1159/000489832
|
[16] |
NAKAWATARI K, KURANO M, ARAKI O, et al. Elevated phosphatidylserine-specific phospholipase A1 level in hyperthyroidism[J]. Clin Chim Acta, 2020, 503(1): 99.
|
[17] |
SAWADA T, KURANO M, SHIRAI H, et al. Serum phosphatidylserine-specific phospholipase A1 as a novel biomarker for monitoring systemic lupus erythematosus disease activity[J]. Int J Rheum Dis, 2019, 22(11): 2059.
|
[18] |
庞杰, 李瑞娟, 张宗芳, 等. SLE患者血清miR-202-3p、PS-PLA1水平与疾病活动度和细胞免疫功能的关系[J]. 山东医药, 2022, 62(13): 40.
|
[19] |
陈庆花, 杨永红, 汪根娥, 等. 早期RA患者血清炎症指标与临床特征、转归的相关性研究[J]. 现代免疫学, 2019, 39(6): 485.
|
[20] |
周颖, 邢嘉翌. 血清抗CCP抗体、IgG-RF检测在RA合并IPF患者中的临床应用价值[J]. 检验医学与临床, 2022, 19(2): 243.
|