Experimental study on LncRNA PVT1 targeting miR-410-3p to regulate the expression of inflammatory and fibrotic components in high glucose-induced human renal tubular epithelial cells

Objective To investigate whether long non-coding RNA(LncRNA) PVT1 targeting miR-410-3p to regulate the expression of inflammatory and fibrotic components in high glucose-induced human renal tubular epithelial cells.

Methods Human renal tubular epithelial cells HK-2 were divided into the blank control(NC) group, high glucose induced(HG) group, si-NC+HG group, si-LncRNA PVT1+HG group, miR-NC+HG group, miR-410-3p+HG group, anti-miR-NC+si-LncRNA PVT1+HG group and anti-miR-410-3p+si-LncRNA PVT1+HG group.The cell activity was detected by CCK-8 assay.The mRNA expression levels of LncRNA PVT1 and miR-410-3p in renal tubular epithelial cells HK-2 were detected by reverse transcription-quantitative polymerase chain reaction, the protein expression levels of fibrosis factor α-smooth muscle actin(α-SMA), fibronectin(Fn), collagen type Ⅰ α1 chain(COL1a1) were detected by Western blotting, and the contents of tumor necrosis factor-α(TNF-α) and interleukin-6(IL-6) were detected by enzyme-linked immunosorbent assay.The interaction between LncRNA PVT1 and miR-410-3p was analyzed by dual luciferase activity assay. Results: The expression level of LncRNA PVT1 in renal tubular epithelial cells HK-2 in the HG group was significantly higher than that in NC group(P < 0.01), the expression level of miR-410-3p and cell activity in the HG group were significantly lower than that in NC group(P < 0.01), and the protein expression levels of α-SMA, Fn, COL1a1, and contents of TNF-α, IL-6 in the HG group were significantly higher than those in the NC group(P < 0.05).After inhibiting the expression of LncRNA PVT1 or overexpressing miR-410-3p, the cell activity of in renal tubular epithelial cells HK-2 increased(P < 0.05), and the protein expression levels of α-SMA, Fn, COL1a1, and contents of TNF-α, IL-6 decreased(P < 0.05).The LncRNA PVT1 targetedly regulated the expression of miR-410-3p.Down-regulating the expression of miR-410-3p could attenuate the inhibitory effects of LncRNA PVT1 expression on α-SMA, Fn, COL1a1 protein expression, TNF-α and IL-6 content in human renal tubular epithelial cells HK-2(P < 0.01).

Conclusions Inhibition of LncRNA PVT1 expression inhibits the expression of inflammatory and fibrotic components in high glucose-induced human renal tubular epithelial cells by targeting miR-410-3p.