Study on the activation of lncRNA LINC00470/PI3K pathway promoting arthrosis autophagy in knee osteoarthritis

Objective: To investigate the effects of lncRNA LINC00470/PI3K pathway activation on the autophagy of bone and joint in knee osteoarthritis(OA) model. Methods: Human articular cartilage samples,OA model rats and IL-1β-treated C28/I2 cells were used in this study.The expression changes of genes and proteins were assessed by real-time quantitative PCR(RT-qPCR) and Western blotting.The cell viability,apoptosis and autophagy were assessed by CCK-8,flow cytometry,immunofluorescence and Western blotting analysis,respectively.Masson staining and TUNEL staining were performed in vivo to evaluate cartilage tissue Mankin score and apoptosis. Results: The LINC00470 was upregulated in OA patients and IL-1β-induced chondrocytes.IL-1β treatment decreased C28/I2 cell viability,increased apoptosis and inhibited autophagy,which were largely reversed by LINC00470 knockdown.The LINC00470 knockdown in C28/I2 cells abolished IL-1β-induced activation of PI3K/AKT/mTOR signaling.In a rat model of OA,LINC00470 knockdown decreased Mankin score,reduced apoptosis,promoted autophagy,and inhibited activation of PI3K/AKT/mTOR signaling. Conclusions: LINC00470 promotes autophagy in OA,which may depend on the PI3K/AKT/mTOR signaling pathway.