Relationship between histone deacetylase 4 expression in peripheral blood and bone mineral density-related indicators in postmenopausal osteoporosis patients

Objective: To investigate the relationship between peripheral blood histone deacetylase 4 (HDAC4) expression in peripheral blood and bone mineral density (BMD)-related indicators in postmenopausal osteoporosis (PMO) patient. Methods: Eighty-two female PMO patients were selected as the observation group,and 82 female health examinees during the same period were selected as the control group.Real time fluorescence quantitative PCR was used to detect the serum HDAC4 mRNA levels;dual energy X-ray absorptiometry was employed to measure the BMD of the subjects;Pearson method was applied to analyze the correlation between serum HDAC4 mRNA expression levels and procollagen type Ⅰ aminoterminal propeptide (PINP),cross-linked C-terminal telopeptide of type Ⅰ collagen (β-CTX),lumbar spine BMD,and femoral neck BMD in PMO patients;logistic regression analysis was used to analyze the influencing factors for PMO occurrence;ROC curve was drawn to evaluate the predictive value of serum HDAC4 mRNA expression level for PMO. Results: Compared with the control group,the levels of serum PINP,lumbar spine BMD,and femoral neck BMD in the observation group were significantly reduced (P<0.01),while the levels of HDAC4 mRNA and β-CTX were significantly increased (P<0.01).Pearson correlation analysis showed that the expression level of serum HDAC4 mRNA in PMO patients was significantly negatively correlated with PINP level,lumbar spine BMD,and femoral neck BMD (r=-0.399,-0.437,-0.492,P<0.01),and significantly positively correlated with β-CTX level (r=0.456,P<0.01).Logistic regression analysis showed that the elevated levels of serum β-TX and HDAC4 mRNA were independent risk factors for PMO (P<0.01).ROC curve analysis showed that the AUC of serum HDAC4 mRNA to predict PMO was 0.878 (95%CI:0.823-0.932),the sensitivity was 80.5%,and the specificity was 94.1%. Conclusions: The expression level of serum HDAC4 mRNA in PMO patients is significantly up-regulated,which is negatively correlated with PINP level and BMD,and positively correlated with β-CTX level.The increased levels of serum β-CTX and HDAC4 mRNA expression are independent risk factors for PMO.