Construction and identification of serial human BRD8 reporter genes

Objective: To construct and identify serial human BRD8 luciferase reporter genes with the identical 3'-end but gradually truncated 5'-end. Methods: The BRD8 promotor fragment from human genome was amplified by polymerase chain reaction(PCR) and inserted into the luciferase reporter gene vector pGL3-Basic. A series of BRD8 promotor fragments with the identical 3'-end but gradually truncated 5'-end were produced by PCR using the above constructed BRD8 reporter gene vector as template and inserted into pGL3-Basic vector,respectively. The series of BRD8 reporter genes were identified by restriction enzyme digestion and sequencing. Results: The restriction enzyme digestion and DNA sequencing results showed that the sequences and orientation of the serial BRD8 reporter genes were correct. Conclusions: The serial human BRD8 reporter genes had been successfully constructed. These provide an experimental base for further research on the regulation of BRD8 gene transcription by some specific transcription factors and/or silencers.