Application of cytochemistry staining in classification of acute leukemia

Objective: To diagnose and classify acute leukemia(AL)by cytochemistry staining.Methods: Bone marrow slides were stained with wright staining and cytochemistry staining including peroxidase(POX),naphthol AS-D chloroacetate esterase(CE),and alpha naphthyl-acetate esterase(NAE)with sodium fluoride inhibition and periodic acid Schiff reaction(PAS).Results: POX,CE and PAS could distinguish acute nonlymphocytic leukemia(ANLL)from acute lymphocytic leukemia(ALL).NAE with sodium fluoride inhibition could differentiate granulocytic leukemia from monocytic leukemia.Furthermore,the diagnostic rate of AL increased from 74.4% with only morphology to 90.1% with both cytochemistry and morphology.Conclusions: Cytochemistry staining is of great value for the diagnosis and classification of AL and can increase the conformity rate.