Culture and identification of rat marrow mesenchymal stem cells

Objective: To culture and identify rat mesenchymal stem cells(MSCs).Methods: Rat MSCs were separated from femur marrow by flushing out with low glucose DMEM,and planted in plastic culture flask.MSCs were observed by means of growth curve and clone forming rates.Vimentin,laminin were detected by immunohistochemistry.Results: The growth curve of MSCs of passage culture as follows:latent period 1-2 days;logarithm period 3-6 days and platform period afterward.Clone forming rates decreased with passage.The cells were purified after fifth passage of MSCs and shown as spindle-shaped morphology.Conclusions: The cultured cells are rat MSCs.The method is easy to operate and simple.