Expressions of IFN-γ-inducible protein-10 and monokine induced by IFN-γ of lung tissue in murine asthmatic model and its significance

Objective: To evaluate the expressions and significance of IFN-γ-inducible protein-10(IP-10) and monokine induced by IFN-γ(Mig) of lung tissue in murine asthmatic model,and to observe the influence of dexamethasone(DX) and bacillus of Calmette and Guerin vaccine(BCG) on the expressions of IP-10 and Mig.Methods: Forty Kunming mice were randomly divided into asthmatic group,DX group,BCG group and control group with 10 in each.The mice in asthmatic group were sensitized and challenged with ovalbumin(OVA) by abdomen injection every other day for 13 days and atomization inhalation of 1%OVA 5 ml from d21 for 7 days to establish asthmatic models.The mice in DX group were administrated DX(2 mg/kg) by abdomen injection 30 min before challenge,the mice in BCG group were injected intradefinally with BCG at day 7,3 and 1 before sensitization and the control group were given normal saline instead of OVA.The expressions of IP-10 and Mig protein of lung tissue in the mice were detected by immunohistochemistry.Results: The IP-10 protein expression in the asthmatic group was significantly higher than that in the control group(P<0.01) but decreased in DX group(P<0.05),and no significant decrement was observed in BCG group(P>0.05).The Mig protein expression in the asthmatic group was significantly lower than that in the control group(P<0.01),and the Mig protein expression in DX group and BCG group were significantly higher than that in asthmatic group(P<0.05).Conclusions: The expressions of IP-10 of lung tissue increased,but Mig decreased in the asthmatic model mice.Dexamethasone may downregulate IP-10 protein expression of lung and upregulate Mig protein expression.BCG may upregulate Mig protein expression of lung,but does not inhibit IP-10 protein expression.The chemokine IP-10 and Mig play an important role in the pathogenesis of asthma.DX and BCG can interfere with the expression of IP-10 and Mig to some extent.