Study on the proportion of peripheval blood T cell subsets and the level of IFN-γ and IL-4 production in patients with cysticercosis

Objective: To detect the proportion of peripheval blood T cell subsets(PBMCs) and the level of IFN-γ/IL-4 production of peripheral blood in patients with cysticercosis,and to investigate the role of T cell subsets in immune regulation of cysticercus cellulosae infection.Methods: The proportions of T-cell subsets of PBMCs and Th1/Th2 cytokines producing cells in patients with cysticercosis were detected by four-color flow cytometry.Cellquest or WinMDI 2.8 was used in analysis of T-cell subsets.Results: The percentage of CD3⁺ and CD4⁺ T-cells subsets,and the ratio of CD4⁺/CD8 in patients with cysticercosis were higher than those of the control(P<0.01).The percentage of secreting IFN-γ/IL-4 CD3⁺ of PBMC in patients with cysticercosis was not higher than that of the control(P>0.05).Conclusions: The patients with cysticercosis have the higher percentages of CD3⁺ and CD4⁺ cells than the control,presenting an imbalance of T cell subsets and abnormality of immunity.