Comparison of IFN- production in human peripheral blood T cell subpopulations stimulated by different antigens from Mycobacterium tuberculosis

Objective:To compare the characteristics and differences of peripheral blood T cells stimulated by HDMAPP,Mycobacterium tuberculosis heat-resistant antigen(Mtb-HAg) and low molecular peptide antigen of Mycobacterium tuberculosis(Mtb)(10 k) to produce interferon(IFN-).Methods:The peripheral blood cells from healthy adults were cultured with HDMAPP and Mtb-HAg and Mtb-HAg(10k) for 14 hours,then added Monensin to cultivate for another 6 hours.Cells were collected,and the surface molecule and intracellular cytokines were stained with different fluorochrome-conjugated mAbs.The expressions of IFN- in CD3+ and CD3+ cells of different experiment group were detected by flow cytometry.Results:The ratios of CD3+ and CD3+ cells to produce IFN- in all antigen experiment groups were higher than that in control group(P0.01).The ratios of CD3+ cells to produce IFN- in all antigen experiment groups were significantly higher than that of CD3+ cells(P0.05 to P0.01).The differences of CD3+ cells and CD3+ cells in three kinds of antigenic stimulation groups were not statistical significance(P0.05).Conclusions:CD3+ cells and CD3+ cells can produce IFN- by stimulating the peripheral blood T cells with HDMAPP,Mtb-HAg and Mtb-HAg(10k).CD3+ cells stimulated by three kinds of antigens can produce IFN-.The IFN- level in CD3+ cells stimulated by HDMAPP and Mtb-HAg(10 k) is higher than that of Mtb-HAg stimulating.