Research on the creation of global cerebral ischemia-reperfusion injury model in mice

Objective: To investigate the success rate and reliability of creating global cerebral ischemia reperfusion injury model by obstructing the bilateral common carolid arteries and pressing the cervix and ventilating the mice in the artificial way and so on.Methods: Artificial respiration was performed using rodent micro-ventilator during the whole experiment course.ECG and EEG were detected carefully.EEG turnning to a straight line was used as the criterion of full cerebral ischemia.Pathological examination was performed in the cerebral cortex and hippocampe.Lactate dehydrogenase (LDH) and creatine phosphokinase(CPK) were determined in the cerebral cortex.Results: EEG turned to a straight line after a few seconds of ischemia and didn't recover within 30 minutes of ishemic and 60 minutes of reperfusion.The LDH and CPK activity of ischemia and reperfusion group was significantly lower than that of control group(P<0.01).CPK activity of reperfusion group was significantly lower than that of ischemia group(P<0.01).Pathologic examination showed that after 30 minutes of ischemia,the cells of cerebral cortex and hippocampe pyramid pyknosed,a small number of neurons degenerated and swelled.The above mentioned damage worsened after 60 minutes of reperfusion.Conclusions: This improved operative method is simple and practical.The global cerebral ischemia-reperfusion injury is obvious in the model,with higher success rate and lower mortality.