Sample factors affecting fluorescence quantitative PCR detection of HBVDNA

Objective: To explore the effects of abnormal sample characters,such as hemolysis,icterus and lipemia,on fluorescence quantitative PCR (FQ-PCR) detection of HBV DNA. Methods: Levels of serum HBV DNA in hyperlipemia with hemolysis,icterus and normal serum samples were detected by FQ-PCR. Results: There were no significant differences of HBV DNA level among the groups of hyperlipemia with hemolysis,icterus and the control (P>0.05). Conclusions: Hemolysis,icterus and lipemia of serum sample have no interference on FQ-PCR detection of HBV DNA.