Detection of drug-resistant genes in meticillin-resistant coagulase negative staphylococcus by PCR assay

Objective: To investigate the seven drug-resistance genes in meticillin-resistant coagulase negative staphylococcus (MRCNS) in Suzhou area. Methods: The drug-resistance genes of mecA,gyrA,qacA/B/C,qacA,ermA/B/C,ermB and TetM were determined with polymerase chain reaction in 133 strains of MRCNS. Results: In the 133 strains of MRCNS,the carrier rates of mecA,gyrA,TetM,qacA/B/C,ermB,qacA and ermA/B/C were 100.0%,47.4%,51.9%,45.1%,25.6%,24.8% and 12.0%,respectively. Conclusions: In Suzhou area of Anhui province,the carrier rates of mecA,qacA/B/C and qacA genes in MRCNS are similar to that of other domestic regions,but that of gyrA,and TetM genes are higher,and ermA/B/C and ermB lower.