Comparison of two methods of handling non-liquefaction semen

Objective: To compare the methods of mechanical mixing and bromelain treatment in handling non-liquefaction semen.Methods: Sixty cases of non-liquefaction semen were taken from the patients in our department and all the sample's volume was ≥1.5 ml after routine examination.Every sample was divided into group A(control group),group B(semen handled by mechanical mixing) and group C(semen treated with bromelain).Human Computer-aided Semen Analysis System of Spain was used to detect the sperm density and motility;at the same time,Eosin Staining was used to detect the sperm viability,and Modified Papanicolaou Staining was employed to analyze the rate of the normal morphology sperm.Results: There was no significant difference among the three groups in the sperm density and viability(P>0.05).The time of semen liquefaction and sperm motility in group B and group C were both significantly higher than those in group A(P<0.01).However,the rate of normal morphology sperm in group B was significantly lower than that in group A and group C(P<0.01).The time of semen liquefaction in group C was significantly shorter than that in group B(P<0.01).Conclusions: Bromelain method is superior to mechanical mixing in handling abnormal liquefaction semen.It can not only shorten the time of semen routine analysis but reduce the semen viscosity,which will avoid sampling error.